›› 2011, Vol. 23 ›› Issue (3): 176-180.doi: 10.3969/j.issn.1004-616x.2011.03.004

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Lipopolysaccharide-preconditioning influences the NO secretion from cultured cerebral astrocytes and microglia in vitro

SU Yun1， XIE Ze-feng2， XIN gang1，XU Yan-xuan1， LI Kang-sheng1，*   

  1. 1. Department of Microbiology and Immunology, Shantou University Medical College, Shantou 515041; 2. The First Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2011-03-21 Revised:2011-04-01 Online:2011-05-30 Published:2011-05-30
  • Contact: LI Kang-sheng

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Abstract: To study the influences of lipopolysaccharide(LPS) preconditioning on NO secretion in cultured cerebral astrocytes and microglia. METHODS： Mice cerebral astrocytes and microglia were isolated and purified in vitro. Thereafter， these glial cells were divided into 4 groups： LPS-primed group， 10 ng/ml LPS treated group， 1 μg/ml LPS treated group and non-treated group. The LPS-primed group was preconditioned with 10 ng/ml of LPS for 18 hours. After the preconditioning， the cells received the second treatment with 1 μg/ml of LPS for 6 hours， 24 hours and 48 hours. Then cultured supernatants of all 4 groups were collected. NO level in the supernatants was determined by Griess method. RESULTS： Both in cultured astrocytes and microglia，NO level of the LPS-primed group was increased at 24 hours with LPS re-treatment compared with the 1 μg/ml LPS-stimulated group (P<0.05). Moreover， at 48 hours with LPS re-treatment， a long-lasting and significant enhancing effect was found in astrocytes (P<0.05). CONCLUSION： LPS preconditioning could enhance NO secretion ability in cultured cerebral astrocytes and microglia.

Key words: astrocyte, microglia, lipopolysaccharide, preconditioning, nitric oxide